bioGenous^TM Human Brain (Differentiation) Organoid Kit is a serum-free culture medium for human brain organoids (hFBs). In human brain organoids expansion medium, hFBs consist of neural stem cells, characterized by the expression of markers such as Sox2. When the expansion medium is replaced with differentiation medium, hFBs can differentiate into matured neuronal cells, which exhibit specific markers including Gfap, Map2, Cux1, and Dcx. Human brain organoids display hallmarks of the human brain in terms of architecture, cell type composition, and self renewal dynamics, therefore hold great promise for studies of human brain development and disease.