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<M1258> Mag-Bind Ultra-Pure Plasmid DNA 96 Kit 磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒

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The Mag-Bind? Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind? technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format.

磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒

Mag-Bind Ultra-Pure Plasmid DNA 96 Kit


產(chǎn)品編號

產(chǎn)品名稱

包裝規(guī)格

目錄價(jià)

M1258-01

     磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒     

4 x 96

3850

M1258-02

磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒

24 x 96

19188


產(chǎn)品介紹

The Mag-Bind? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek’s proprietary Mag-Bind? Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

The Mag-Bind? Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind? technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye? sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.


參數(shù)

FEATURES

SPECIFICATIONS

Downstream Application

Sensitive cell line transfection; cloning; sanger sequencing etc.

Starting material

Per well: 1.0–1.5 mL LB culture with OD600 between 2 and 3; or equivalent

Plasmid type

High-copy, low-copy, cosmid DNA

Processing mode

Automated, Magnetic processing

Throughput

96 samples per run

DNA binding technology

Magnetic beads

Lysate clearance method

Centrifugation, Magnetic Beads or 96-well Filter Plate

Yield

10–15 μg for high copy-number plasmid


組分

ProductNo.

M1258-00

M1258-01

Purification

1 × 96

4 × 96

Mag-Bind? Particles RQ

2.2 mL

8.8 mL

Solution I

30 mL

120 mL

Solution II

30 mL

120 mL

N3 Buffer

15 mL

60 mL

ETR Binding Buffer

60 mL

250 mL

ETR Wash Buffer

60 mL

250 mL

VHB Buffer

66 mL

264 mL

SPM Buffer

15 mL

60 mL

Elution Buffer

15 mL

60 mL

RNase A

50 μL

200 μL

User Manual

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