磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒
Mag-Bind Ultra-Pure Plasmid DNA 96 Kit
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝規(guī)格 | 目錄價(jià) |
M1258-01 | 磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒 | 4 x 96 | 3850 |
M1258-02 | 磁珠法無內(nèi)毒素質(zhì)粒小量提取試劑盒 | 24 x 96 | 19188 |
產(chǎn)品介紹
The Mag-Bind? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek’s proprietary Mag-Bind? Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.
The Mag-Bind? Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind? technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye? sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.
參數(shù)
FEATURES | SPECIFICATIONS |
Downstream Application | Sensitive cell line transfection; cloning; sanger sequencing etc. |
Starting material | Per well: 1.0–1.5 mL LB culture with OD600 between 2 and 3; or equivalent |
Plasmid type | High-copy, low-copy, cosmid DNA |
Processing mode | Automated, Magnetic processing |
Throughput | 96 samples per run |
DNA binding technology | Magnetic beads |
Lysate clearance method | Centrifugation, Magnetic Beads or 96-well Filter Plate |
Yield | 10–15 μg for high copy-number plasmid |
組分
ProductNo. | M1258-00 | M1258-01 |
Purification | 1 × 96 | 4 × 96 |
Mag-Bind? Particles RQ | 2.2 mL | 8.8 mL |
Solution I | 30 mL | 120 mL |
Solution II | 30 mL | 120 mL |
N3 Buffer | 15 mL | 60 mL |
ETR Binding Buffer | 60 mL | 250 mL |
ETR Wash Buffer | 60 mL | 250 mL |
VHB Buffer | 66 mL | 264 mL |
SPM Buffer | 15 mL | 60 mL |
Elution Buffer | 15 mL | 60 mL |
RNase A | 50 μL | 200 μL |
User Manual | ? | ? |