使用磁珠法從PCR及酶切產物中分離高濃度的DNA片段

Mag-Bind RXNPure Plus

 產品介紹

The Mag-Bind? RxnPure Plus Kit allows rapid and reliable isolation DNA from PCR* and other enzymatic reactions with high yield. The system combines Omega Bio-tek’s proprietary DNA extraction chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger and eliminate excess nucleotides, primers, and nonspecific products such as primer dimers. This kit is designed for both manual and fully automated protocols and may not require reprogramming of liquid handling instruments depending on your current method. The Mag-Bind? RxnPure Plus is compatible with multiple liquid handlers and magnetic processors such as Hamilton STAR/STARlet and Beckman Coulter Biomek FX/NX.

• Removes excess primers, primer-dimers, dNTPs and salts

• Does not require centrifugation/filtration steps

• Can be adapted and scaled to most standard liquid handling robots

• Available in 96- or 384- well format

1. Measure the PCR Reaction

2. Add Mag-Bind? RXNPure Plus and Mix

3. Magnetize and Remove Supernatant

4. Wash Twice with 70% Ethanol

5. Dry

6. Elute DNA