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<D2492> Blood DNA Maxi Kit 血液DNA大提試劑盒

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The E.Z.N.A.? Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used.

血液DNA大提試劑盒
Blood DNA Maxi Kit


產(chǎn)品編號

產(chǎn)品名稱

包裝規(guī)格

目錄價

D2492-01

血液DNA大提試劑盒     

5T

450

D2492-02

血液DNA大提試劑盒

20T

1710

D2492-03

血液DNA大提試劑盒

50T

3600

產(chǎn)品介紹

The E.Z.N.A.? Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.? Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.


參數(shù)

FEATURES

SPECIFICATIONS

Starting Amount

Up to 20 mL

Starting Material

Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat

Yield

2 mg

Elution Volume

2000 μL

Technology

HiBind? DNA Maxi column

Processing Mode

Manual, centrifugation/vaccuum

Throughput

1–24


組分

Product

D2492-03

Preparations

50

HiBind? DNA Maxi Columns

50

50 mL Collection Tubes

50

TL Buffer

375 mL

BL Buffer

600 mL

HBC Buffer

200 mL

DNA Wash Buffer

2 × 200 mL

RNase A

1.1 mL

Proteinase K Solution

13 mL

Elution Buffer

500 mL

User Manual

?


流程

  1. Lysis

  2. Adjust Binding Conditions

  3. Bind

  4. Wash

  5. Elute