<D3396> Tissue DNA Kit 組織DNA提取試劑盒

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組織DNA提取試劑盒

Tissue DNA Kit

產品編號	產品名稱	包裝規格	目錄價
D3396-00	組織DNA提取試劑盒	5T	126
D3396-01	組織DNA提取試劑盒	50T	720
D3396-02	組織DNA提取試劑盒	200T	2610

產品介紹

The E.Z.N.A. Tissue DNA Kit offers a versatile and cost-effective method for isolating DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with Silica Mini Spin Column technology into four quick lyse, bind, wash, and elute steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation and the use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis, and restriction enzyme digestion.

參數

FEATURES	SPECIFICATIONS
Downstream Applications	PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion
Starting Material	Tissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs
Starting Amount	30 mg, or 5 × 10? cultured cells
Elution Volume	100–200 μL
DNA Binding Technology	Silica mini spin column
Throughput	1–24
Processing Time	< 20 min (post-lysis)

組分

Product	D3396-00	D3396-01	D3396-02	D3396-03
Purifications	5	50	200	600
HiBind? DNA Mini Columns	5	50	200	600
2 mL Collection Tubes	10	100	400	1200
BL Buffer	5 mL	20 mL	60 mL	2 × 100 mL
TL Buffer	5 mL	20 mL	60 mL	135 mL
HBC Buffer	5 mL	25 mL	80 mL	250 mL
DNA Wash Buffer	2.5 mL	25 mL	3 × 25 mL	200 mL
Elution Buffer	15 mL	30 mL	2 × 60 mL	300 mL
Proteinase K Solution	150 μL	1.5 mL	6 mL	18 mL
User Manual	?	?	?	?

流程

Lyse
Adjust Binding Conditions
Bind
Wash 3X
Dry
Elute

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<D3396> Tissue DNA Kit 組織DNA提取試劑盒