96孔磁珠法糞便DNA提取試劑盒
Mag-Bind Stool DNA 96 Kit
產品編號 | 產品名稱 | 包裝規格 | 目錄價 |
M4016-00 | 96孔磁珠法糞便DNA提取試劑盒 | 1 x 96 | 2550 |
M4016-01 | 96孔磁珠法糞便DNA提取試劑盒 | 4 x 96 | 8800 |
產品介紹
The Mag-Bind Stool DNA 96 Kit allows for rapid and reliable isolation of high-quality host as well as pathogenic genomic DNA from stool samples. Up to 300 μL stool samples can be processed in less than 45 minutes. The kit includes our 96-well disruptor plates, which are pre-filled with glass beads. The protocol involves no organic extractions, reducing both plastic waste and hands-on time to allow parallel processing of multiple samples. Purified DNA is inhibitor-free and is suitable for various downstream applications such as PCR, restriction digestion, and NGS.
The Mag- Bind? technology is ideally suited for automated liquid handlers, and the uniquely formulated cHTR reagent eliminates PCR-inhibiting compounds such as humic acids, lipids, etc., commonly found in stool samples. The extraction system allows for automation after sample lysis via Hamilton Microlab? STAR?, Thermo Fisher Scientific KingFisher? Flex, Applied Biosystems MagMAX? 96, Qiagen BioSprint? 96, and other liquid handling instruments.
參數
FEATURES | SPECIFICATIONS |
Starting Amount | 300 μL |
Starting Material | Stool |
Elution Volume | 50–100 μL |
Technology | Magnetic beads |
Processing Mode | Automated, Manual |
Throughput | 96 |
組分
ProductNumber | M4016-00 | M4016-01 |
Purifications | 1 × 96 preps | 4 × 96 preps |
E-Z 96 Disruptor Plate C with Caps | 1 | 4 |
SLX-Mlus Buffer | 60 mL | 240 mL |
DS Buffer | 5 mL | 45 mL |
SP2 Buffer | 12 mL | 45 mL |
XP2 Binding Buffer | 60 mL | 250 mL |
VHB Buffer | 22 mL | 88 mL |
SPM Buffer | 30 mL | 4 × 30 mL |
Elution Buffer | 30 mL | 60 mL |
Proteinase K Solution | 2.2 mL | 8.8 mL |
RNase A | 220 μL | 880 μL |
Mag-Bind? Particles CH | 2.2 mL | 8.8 mL |
cHTR Reagent | 12 mL | 45 mL |
User Manual | ? | ? |
流程
Lyse Samples
Precipitate Proteins and Humic Acids
Transfer Supernatant, Adjust Binding Conditions, Add Magnetic Beads to Bind
Concentrate Magnetic Beads, Discard Supernatant
Wash Magnetic Beads Twice
Concentrate Magnetic Beads, Discard Supernatant
Dry
Elute
Transfer Product