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<M4016>Mag-Bind Stool DNA 96 Kit 96孔磁珠法糞便DNA提取試劑盒

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The Mag-Bind Stool DNA 96 Kit allows for rapid and reliable isolation of high-quality host as well as pathogenic genomic DNA from stool samples.

96孔磁珠法糞便DNA提取試劑盒

Mag-Bind Stool DNA 96 Kit 


產品編號

產品名稱

包裝規格

目錄價

M4016-00

    96孔磁珠法糞便DNA提取試劑盒      

1 x 96

2550

M4016-01

96孔磁珠法糞便DNA提取試劑盒  

4 x 96

8800


產品介紹

The Mag-Bind Stool DNA 96 Kit allows for rapid and reliable isolation of high-quality host as well as pathogenic genomic DNA from stool samples. Up to 300 μL stool samples can be processed in less than 45 minutes. The kit includes our 96-well disruptor plates, which are pre-filled with glass beads. The protocol involves no organic extractions, reducing both plastic waste and hands-on time to allow parallel processing of multiple samples. Purified DNA is inhibitor-free and is suitable for various downstream applications such as PCR, restriction digestion, and NGS.

The Mag- Bind? technology is ideally suited for automated liquid handlers, and the uniquely formulated cHTR reagent eliminates PCR-inhibiting compounds such as humic acids, lipids, etc., commonly found in stool samples. The extraction system allows for automation after sample lysis via Hamilton Microlab? STAR?, Thermo Fisher Scientific KingFisher? Flex, Applied Biosystems MagMAX? 96, Qiagen BioSprint? 96, and other liquid handling instruments.


參數

FEATURES

SPECIFICATIONS

Starting Amount

300 μL

Starting Material

Stool

Elution Volume

50–100 μL

Technology

Magnetic beads

Processing Mode

Automated, Manual

Throughput

96


組分

ProductNumber

M4016-00

M4016-01

Purifications

1 × 96 preps

4 × 96 preps

E-Z 96 Disruptor Plate C with Caps

1

4

SLX-Mlus Buffer

60 mL

240 mL

DS Buffer

5 mL

45 mL

SP2 Buffer

12 mL

45 mL

XP2 Binding Buffer

60 mL

250 mL

VHB Buffer

22 mL

88 mL

SPM Buffer

30 mL

4 × 30 mL

Elution Buffer

30 mL

60 mL

Proteinase K Solution

2.2 mL

8.8 mL

RNase A

220 μL

880 μL

Mag-Bind? Particles CH

2.2 mL

8.8 mL

cHTR Reagent

12 mL

45 mL

User Manual

?

?


流程

  1. Lyse Samples

  2. Precipitate Proteins and Humic Acids

  3. Transfer Supernatant, Adjust Binding Conditions, Add Magnetic Beads to Bind

  4. Concentrate Magnetic Beads, Discard Supernatant

  5. Wash Magnetic Beads Twice

  6. Concentrate Magnetic Beads, Discard Supernatant

  7. Dry

  8. Elute

  9. Transfer Product