磁珠法石蠟包埋組織DNA提取試劑盒
Mag-Bind FFPE DNA Kit
產品編號 | 產品名稱 | 包裝規格 | 目錄價 |
M6957-01 | 磁珠法石蠟包埋組織DNA提取試劑盒 | 50T | 3250 |
M6958-01 | 磁珠法石蠟包埋組織DNA提取試劑盒 | 4 x 96 | 17390 |
產品介紹
The Mag-Bind? FFPE DNA 96 Kit is designed for rapid and reliable isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. The specially formulated buffers reverse crosslinking and release short and long DNA fragments. Mag-Bind? Particles allow for automation-friendly extraction from FFPE tissue in under 6 hours. Purified DNA is suitable for downstream applications including SNP analysis, sequencing, and genotyping. This system is fully compatible on Hamilton Star, Beckman Coulter Biomek? FX, Biomek? 2000, Tecan Fluent?, Thermo KingFisher? Flex, and other workstations.
參數
FEATURES | SPECIFICATIONS |
Starting Amount | 3–8 paraffin sample sections between 5–10 μm |
Starting Material | FFPE tissue |
Elution Volume | 30–50 μL |
Technology | Magnetic beads |
Processing Mode | Automated, Manual |
Throughput | 96 |
組分
Mag-Bind?FFPE DNA 96 Kit | M6958-00 | M6958-01 |
Preparations | 1 × 96 preps | 4 × 96 preps |
Mag-Bind? Particles CH | 3.3 mL | 14 mL |
FTL2 Buffer | 30 mL | 110 mL |
MB4 Buffer | 75 mL | 250 mL |
MPWP Buffer | 30 mL | 125 mL |
DNA Wash Buffer | 50 mL | 200 mL |
LPA | 1.1 mL | 4.4 mL |
Proteinase K Solution | 2.2 mL | 9 mL |
Elution Buffer | 30 mL | 60 mL |
User Manual | ? | ? |
流程
Heat Incubate for Lysis / Decrosslink at 90℃
Transfer Supernatant / Adjust Binding Conditions
Bind Nucleic Acids with Magnetic Beads
Concentrate Magnetic Beads
Wash
Concentrate Magnetic Beads
Dry
Elute
Concentrate Magnetic Beads / Transfer Product