欧美大荫蒂xxxI午夜少妇一区二区三区I精品一区二区三区在线播放I婷婷日日I狠狠色噜噜狠狠狠合久I久久夜靖品I成人网看片I最近日本韩国中文字幕I日本精品视频在线I日韩欧美国产精品

<M3298> Mag-Bind cfDNA Kit 磁珠法游離DNA提取試劑盒

分享到：
The Mag-Bind? cfDNA Kit is designed for the rapid and efficient isolation of circulating cell-free DNA from up to 10 mL plasma or serum samples. The Mag-Bind cfDNA Kit can be processed manually or using automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

磁珠法游離DNA提取試劑盒

Mag-Bind cfDNA Kit


產品編號

產品名稱

包裝規格

目錄價

M3298-00

   磁珠法游離DNA提取試劑盒     

5T

580

M3298-01

磁珠法游離DNA提取試劑盒  

50T

4600

M3298-02

 磁珠法游離DNA提取試劑盒   

200T

18000


產品介紹

The Mag-Bind? cfDNA Kit is designed for the rapid and efficient isolation of circulating cell-free DNA from up to 10 mL plasma or serum samples. The Mag-Bind cfDNA Kit can be processed manually or using automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

The unique formulation of the lysis and binding buffers allows complete automation of the extraction process for up to 10 mL sample volumes with minimal user intervention. The magnetic properties of the Mag-Bind Particles CH enable fast magnetic separation, even when using large volumes. The high binding capacity of the beads allows for a lower volume of magnetic particles needed, thus reducing the final elution volume required. 10 mL of serum or plasma can be eluted in as low as 50 μL.

The system combines the reversible nucleic acid-binding properties of Mag-Bind paramagnetic particles with a unique binding system that targets smaller DNA fragments (150-400 bp) and minimizes the binding of larger fragments, such as gDNA.


參數

FEATURES

SPECIFICATIONS

Sample Type

Plasma/Serum

Sample Volume

500–10,000 μL

Technology

Magnetic Beads

Processing

Manual or Automated

Automatable

Yes

Processing Time

2 hours for 96 (1mL) samples

Format

Tube, 24-well, 96-well

Elution Volume

50 μL


組分

Product

M3298-00

M3298-01

M3298-02

Preps

5

50

200

DS Buffer

1.5 mL

20 mL

80 mL

JSB Buffer

25 mL

9 × 25 mL

4 × 220 mL

GT7 Buffer v1.1

11 mL

110 mL

2 × 220 mL

SPW Buffer

2.5 mL

25 mL

2 × 50 mL

Elution Buffer

30 mL

250 mL

2 × 250 mL

Proteinase K Solution

350 μL

4 mL

14 mL

Mag-Bind? Particles CH

110 μL

1.1 mL

4.4 mL

User Manual

?

?

?


流程

cfDNA Extraction and Purification from up to 1 mL Serum/Plasma

  1. Add up to 1 mL serum/plasma sample to a 15 mL centrifuge tube (not provided). Bring volume up to 1 mL with Elution Buffer if sample volume is less than 1 mL.

  2. Add 15 μL Proteinase K Solution and 67 μL D5 Buffer sequentially. Vortex at maximum speed or pipet up and down to mix thoroughly.

  3. Incubate at 60°C for 20 minutes. Mix by inverting or shaking every 10 minutes. Let sit at room temperature for 10 minutes.

  4. Add 1 mL JSB Buffer and 5 μL Mag-Bind? Particles CH. Vortex at maximum speed for 30 seconds or pipet up and down to mix thoroughly.

  5. Invert the sample 10 times or pipet up and down to mix. Let sit for 10 minutes at room temperature with continuous mixing. The samples must be mixed throughout the 10 minute incubation period by shaking or rocking. Do not vortex at high speeds as this will cause excess foaming that can reduce yield. The speed of mixing should be set to continuously keep the Mag-Bind? Particles CH suspended in solution.

  6. Transfer 1 mL lysate to a 1.5 mL microcentrifuge tube (not provided). Place the tube on a magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are completely cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH. Remove the tube from the magnetic separation device.

  7. Transfer the remaining lysate from Step 5 to the 1.5 mL microcentrifuge tube used in the previous step. Place the tube on a magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are completely cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH. Remove the tube from the magnetic separation device.

  8. Add 500 μL GT7 Buffer v1.1. Vortex for 2 minutes to resuspend the Mag-Bind? Particles CH. Complete resuspension of the Mag-Bind? Particles CH is critical for obtaining good purity.

  9. Place the tube on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH have cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH. Remove the tube from the magnetic separation device.

  10. Repeat Steps 8-9 for a second GT7 Buffer v1.1 step.

  11. Add 500 μL SPW Buffer diluted with 100% ethanol (see bottle for instructions). Vortex for 2 minutes to resuspend the Mag-Bind? Particles CH. Complete resuspension of the Mag-Bind? Particles CH is critical for obtaining good purity.

  12. Place the tube on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH have cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH.

  13. Remove the tube from the magnetic separation device.

  14. Repeat Steps 11-12 for a second SPW Buffer step.

  15. Remove the tube from the magnetic separation device for approximately 30 seconds. Place the tube on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Aspirate and discard residual SPW Buffer.

  16. Leave the tube on the magnetic separation device for 25 minutes to dry the Mag-Bind? Particles CH. Remove the tube containing the Mag-Bind? Particles CH from the magnetic separation device.

  17. Add 30-60 μL Elution Buffer. Vortex at room temperature for 5 minutes to resuspend the Mag-Bind? Particles CH.

  18. Place the tube on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are completely cleared from solution.

  19. Transfer the cleared supernatant containing the purified DNA to a 1.5 mL microcentrifuge tube or clean microplate (not provided). Store DNA at -20°C.


cfDNA Extraction and Purification from up to 4 mL Serum/Plasma

  1. Add up to 4 mL serum/plasma sample to a 15 mL centrifuge tube (not provided) or 24-well deep-well plate (not provided). Choose the correct plasticware depending on the magnetic separation device being used. Bring volume up to 4 mL with Elution Buffer if sample volume is less than 4 mL.

  2. Add 60 μL Proteinase K Solution and 270 μL D5 Buffer sequentially. Vortex at maximum speed or pipet up and down to mix thoroughly.

  3. Incubate at 60°C for 30 minutes. Mix by inverting or shaking every 10 minutes. Let sit at room temperature for 10 minutes.

  4. Add 4 mL JSB Buffer and 20 μL Mag-Bind? Particles CH. Vortex at maximum speed for 30 seconds or pipet up and down to mix thoroughly.

  5. Invert the sample 10 times or pipet up and down to mix. Let sit for 10 minutes at room temperature with continuous mixing. The samples must be mixed throughout the 10 minute incubation period by shaking or rocking. Do not vortex at high speeds as this will cause excess foaming that can reduce yield. The speed of mixing should be set to continuously keep the Mag-Bind? Particles CH resuspended in solution.

  6. Place the tube/plate on a magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are cleared completely from solution. Aspirate and discard the cleared supernatant. Do not disturb the particles. Remove the tube/plate from the magnetic separation device.

  7. Add 1 mL GT7 Buffer v1.1. Vortex 5 minutes to resuspend the Mag-Bind? Particles CH. Complete resuspension of the Mag-Bind? Particles CH is critical for obtaining good purity.

  8. Transfer the resuspended Mag-Bind? Particles CH to a new 1.5 mL microcentrifuge tube (not provided) if using a 15 mL centrifuge tube for Steps 1-7. Use a magnetic separation device designed for 1.5/2.0 mL tubes for the remaining procedure. If using a 24-well deep-well plate for Steps 1-7, continue to use the 24-well deep-well plate and a 24-well magnet.

  9. Place the tube/plate on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH. Remove the tube/plate from the magnetic separation device.

  10. Add another 1 mL GT7 Buffer v1.1. Vortex 5 minutes to resuspend the Mag-Bind? Particles CH. Complete resuspension of the Mag-Bind? Particles CH is critical for obtaining good purity.

  11. Place the tube/plate on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH. Remove the tube/plate from the magnetic separation device.

  12. Add 1 mL SPW Buffer diluted with 100% ethanol (see bottle for instructions). Vortex for 5 minutes to resuspend the Mag-Bind? Particles CH.

  13. Place the tube/plate on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are completely cleared from solution. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind? Particles CH.

  14. Remove the tube/plate from the magnetic separation device.

  15. Repeat Steps 12-13 for a second SPW Buffer step.

  16. Remove the tube/plate from the magnetic separation device for approximately 30 seconds. Place the tube/plate back on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Aspirate and discard the residual SPW Buffer.

  17. Leave the tube/plate on the magnetic separation device for 25 minutes to dry the Mag-Bind? Particles CH. Remove the tube/plate containing the Mag-Bind? Particles CH from the magnetic separation device.

  18. Add 50-100 μL Elution Buffer. Vortex at room temperature for 5 minutes to resuspend the Mag-Bind? Particles CH.

  19. Place the tube/plate on the magnetic separation device to magnetize the Mag-Bind? Particles CH. Let sit at room temperature until the Mag-Bind? Particles CH are completely cleared from solution.

  20. Transfer the cleared supernatant containing purified DNA to a 1.5 mL microcentrifuge tube or clean microplate (not provided). Store DNA at -20°C.