<R6812> HP Total RNA Kit HP組織RNA提取試劑盒

分享到：

HP組織RNA提取試劑盒

HP Total RNA Kit

產(chǎn)品編號(hào)	產(chǎn)品名稱(chēng)	包裝規(guī)格	目錄價(jià)
R6812-00	組織RNA提取試劑盒	5T	150
R6812-01	組織RNA提取試劑盒	50T	1200
R6812-02	組織RNA提取試劑盒	200T	3800

產(chǎn)品介紹

The E.Z.N.A.? HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind? Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.? HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

參數(shù)

FEATURES	SPECIFICATIONS
Downstream Application	RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Starting Material	Cultured cells and tissues
Starting Amount	1 × 10? eukaryotic cells or 25–30 mg tissue
Elution Volume	40–70 μL
RNA Binding Technology	Silica Mini spin column
Processing Mode	Manual (centrifugation or vacuum)
Throughput	1 – 24
Final Purified Product	Total RNA (> 200 nt)
RNA Yield	Up to 100 μg
Processing Time	25 min
Special Notes	RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step

組分

Product	R6812-00	R6812-01	R6812-02
Preparations	5	50	200
HiBind? RNA Mini Columns	5	50	200
RNA Homogenizer Mini Columns	5	50	200
2 mL Collection Tubes	10	100	400
GTC Lysis Buffer	5 mL	40 mL	150 mL
RNA Wash Buffer I	5 mL	50 mL	200 mL
RNA Wash Buffer II	5 mL	12 mL	50 mL
Nuclease-free Water	2 mL	15 mL	60 mL
User Manual	?	?	?