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<D2500> Gel Extraction Kit (V-spin) 膠回收試劑盒

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The E.Z.N.A. Gel Extraction Kit uses spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%).

膠回收試劑盒

Gel Extraction Kit (V-spin)


產品編號

產品名稱

包裝規格

目錄價

D2500-00

     膠回收試劑盒     

5T

詢價

D2500-01

膠回收試劑盒

50T

詢價

D2500-02

膠回收試劑盒

200T

詢價


產品介紹

The E.Z.N.A. Gel Extraction Kit uses spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps, and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.


  • Rapid – DNA recovery from an agarose gel in 15 min

  • Specialized buffer system – Visual determination of optimum DNA binding for higher yields

  • Safe – No phenol/chloroform extractions

  • Versatile – Spin and vacuum formats available

  • High-quality – DNA is suitable for a variety of downstream applications


參數

FEATURES

SPECIFICATIONS

Downstream application

Cloning,in Vitro Transcription,Nucleic Acid Labeling,PCR,Real-time Quantitative PCR(qPCR),Sequencing,Southern Blotting

Elution volume

30–50 μL

Starting material

Agarose gel slice

Starting amount

Up to 25 μg DNA

DNA recovered

>85% recovery, 70 bp to 20 kb

Processing mode

Manual, centrifugation/vacuum

Throughput

1–12

DNA binding technology

Silica mini spin column

Binding capacity

25 μg

Processing time

10 minutes


組分

Product

D2500-00

D2500-01

D2500-02

Purifications

5

50

200

HiBind? DNA Mini Columns

5

50

200

2 mL Collection Tubes

5

50

200

XP2 Binding Buffer

5 mL

40 mL

150 mL

SPW Buffer

5 mL

25 mL

3 × 25 mL

Elution Buffer

2 mL

30 mL

30 mL

User Manual

?

?

?


流程

  1. Excise DNA Band from Gel

  2. Incubate and Melt Gel with Binding Buffer

  3. Transfer Lysate & Bind

  4. Wash 3x

  5. Dry

  6. Elute