膠回收試劑盒
Gel Extraction Kit (V-spin)
產品編號 | 產品名稱 | 包裝規格 | 目錄價 |
D2500-00 | 膠回收試劑盒 | 5T | 詢價 |
D2500-01 | 膠回收試劑盒 | 50T | 詢價 |
D2500-02 | 膠回收試劑盒 | 200T | 詢價 |
產品介紹
The E.Z.N.A. Gel Extraction Kit uses spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps, and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
Rapid – DNA recovery from an agarose gel in 15 min
Specialized buffer system – Visual determination of optimum DNA binding for higher yields
Safe – No phenol/chloroform extractions
Versatile – Spin and vacuum formats available
High-quality – DNA is suitable for a variety of downstream applications
參數
FEATURES | SPECIFICATIONS |
Downstream application | Cloning,in Vitro Transcription,Nucleic Acid Labeling,PCR,Real-time Quantitative PCR(qPCR),Sequencing,Southern Blotting |
Elution volume | 30–50 μL |
Starting material | Agarose gel slice |
Starting amount | Up to 25 μg DNA |
DNA recovered | >85% recovery, 70 bp to 20 kb |
Processing mode | Manual, centrifugation/vacuum |
Throughput | 1–12 |
DNA binding technology | Silica mini spin column |
Binding capacity | 25 μg |
Processing time | 10 minutes |
組分
Product | D2500-00 | D2500-01 | D2500-02 |
Purifications | 5 | 50 | 200 |
HiBind? DNA Mini Columns | 5 | 50 | 200 |
2 mL Collection Tubes | 5 | 50 | 200 |
XP2 Binding Buffer | 5 mL | 40 mL | 150 mL |
SPW Buffer | 5 mL | 25 mL | 3 × 25 mL |
Elution Buffer | 2 mL | 30 mL | 30 mL |
User Manual | ? | ? | ? |
流程
Excise DNA Band from Gel
Incubate and Melt Gel with Binding Buffer
Transfer Lysate & Bind
Wash 3x
Dry
Elute