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<D6294> MicroElute Cycle-Pure&Gel Extraction Kit 微量膠回收試劑盒

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The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources.

微量膠回收試劑盒

MicroElute Cycle-Pure&Gel Extraction Kit 


產(chǎn)品編號

產(chǎn)品名稱

包裝規(guī)格

目錄價

D6294-00

     微量膠回收試劑盒     

5T

100

D6294-01

微量膠回收試劑盒

50T

400

D6294-02

微量膠回收試劑盒

200T

1500


產(chǎn)品介紹

The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind? matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer. The MicroElute? Clean Up system, designed for rapid DNA clean up, includes:

? MicroElute? Cycle-Pure – for direct purification of double or single stranded PCR products (100 bp – 10 kb) from amplification reactions
? MicroElute? Gel Extraction – for extraction of DNA fragments from standard, or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer


參數(shù)

FEATURES

SPECIFICATIONS

Downstream application

Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting

Elution volume

10–15 μL

Starting material

Agrose gel slice

Starting amount

Up to 10 μg DNA

DNA recovered

>90% recovery, 70 bp to 20 kb

Processing mode

Manual, centrifugation/vacuum

Throughput

1

DNA binding Technology

Silica MicroElute spin column

Binding capacity

10 μg

Processing time

<15 minutes

Special note

Low elution volume


組分

Product

D6294-00

D6294-01

D6294-02

Purifications

5

50

200

MicroElute? LE DNA Columns

5

50

200

2 mL Collection Tubes

5

50

200

CP Buffer

5 mL

30 mL

120 mL

XP2 Binding Buffer

5 mL

30 mL

120 mL

Buffer HB

5 mL

25 mL

25 mL

SPW Wash Buffer

2.5 mL

25 mL

3 × 25 mL

Elution Buffer

2 mL

30 mL

30 mL

User Manual

?

?

?


流程

  1. Excise Gel Slice

  2. Dissolve Gel

  3. Bind DNA

  4. Wash

  5. Dry Spin

  6. Elute DNA