產品介紹

The Mag-BIND? Bacterial DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0.5 mL gram positive or gram negative bacterial culture can be processed each time. The key to the system is Omega Bio-tek’s proprietary Mag-BIND? particles that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. After bacterial cells are collected from culture or picked from an agar plate, the bacterial cell wall is removed by lysozyme digestion, followed by Proteinase K digestion. Following lysis, binding conditions are adjusted and the sample is mixed with Mag-BIND particles to bind DNA. Three rapid wash steps remove trace salts and protein contaminants, and finally, DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

參數

組分

流程

1. Harvest bacterial culture

2. Lyse cells with lysozyme

3. Transfer supernatant and bind nucleic acids to magnetic beads

4. Concentrate magnetic beads

5. Wash and dry

6. Elute

7. Concentrate beads and transfer eluate