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<D0926> Insect DNA Kit 昆蟲DNA提取試劑盒

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The E.Z.N.A.? Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples.

昆蟲DNA提取試劑盒

Insect DNA Kit


產品編號

產品名稱

包裝規格

目錄價

D0926-00

    昆蟲DNA提取試劑盒      

5T

135

D0926-01

昆蟲DNA提取試劑盒  

50T

810

D0926-02

昆蟲DNA提取試劑盒  

200T

3150


產品介紹

The E.Z.N.A.? Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.



參數

FEATURES

SPECIFICATIONS

Starting Amount

50 mg

Starting Material

Insect tissue

Yield

100 μg

Elution Volume

50–100 μL

Technology

HiBind? DNA Mini Column

Processing Mode

Manual

Throughput

1–24


組分

Product

D0926-00

D0926-01

D0926-02

Purifications

5

50

200

HiBind? DNA Mini Columns

5

50

200

2 mL Collection Tubes

10

100

400

CTL Buffer

2 mL

20 mL

80 mL

CBL Buffer

3 mL

30 mL

120 mL

HBC Buffer

3 mL

25 mL

80 mL

Proteinase K Solution

150 μL

1.4 mL

4 × 1.4 mL

RNase A

12 μL

120 μL

450 μL

DNA Wash Buffer

1.5 mL

15 mL

3 × 20 mL

Elution Buffer

1 mL

20 mL

50 mL

User Manual

?

?

?


流程

  • Lyse

  • Extract with chloroform

  • Bind

  • Wash

  • Dry

  • Elute